antibodies targeting slc8a1  (Proteintech)

Journal: Molecular Cytogenetics

Article Title: SLC8A1 as a novel susceptibility gene in facilitating tendinopathy: insights into its mechanisms from Mendelian randomization and experimental validation

doi: 10.1186/s13039-025-00738-z

Figure Lengend Snippet: MR analysis demonstrated that genetic susceptibility to SLC8A1 eQTL may accelerate TD progression

Article Snippet: The sections were permeabilized with 0.1% Triton X-100 in PBS for 15 min. After permeabilization, the sections were blocked using a 5% bovine serum albumin solution at ambient temperature for 1 h. The sections were incubated overnight with primary antibodies targeting SLC8A1 (1:1000, 28447-1-AP, Proteintech, Wuhan, China).

Techniques:

Journal: Molecular Cytogenetics

Article Title: SLC8A1 as a novel susceptibility gene in facilitating tendinopathy: insights into its mechanisms from Mendelian randomization and experimental validation

doi: 10.1186/s13039-025-00738-z

Figure Lengend Snippet: Transcriptome sequencing data analysis confirmed that genetic susceptibility to SLC8A1 eQTL contributed to TD progression

Article Snippet: The sections were permeabilized with 0.1% Triton X-100 in PBS for 15 min. After permeabilization, the sections were blocked using a 5% bovine serum albumin solution at ambient temperature for 1 h. The sections were incubated overnight with primary antibodies targeting SLC8A1 (1:1000, 28447-1-AP, Proteintech, Wuhan, China).

Techniques: Sequencing

Journal: Molecular Cytogenetics

Article Title: SLC8A1 as a novel susceptibility gene in facilitating tendinopathy: insights into its mechanisms from Mendelian randomization and experimental validation

doi: 10.1186/s13039-025-00738-z

Figure Lengend Snippet: The experiments verified that SLC8A1 promoted progression of TD. ( A ) Photos of the Achilles tendon in control and tendinopathy groups. ( B ) H&E and Masson’s staining for control and TD tendon. Scale bar: 200 μm. ( C ) Immunofluorescence staining for SLC8A1 in control and TD tissues. Scale bar: 100 μm. ( D ) Western blotting was used to detect SLC8A1 protein levels in tendon tissues of the normal group and TD group. ( E ) qRT-PCR analysis of the mRNA expression of SLC8A1 in TD groups. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: The sections were permeabilized with 0.1% Triton X-100 in PBS for 15 min. After permeabilization, the sections were blocked using a 5% bovine serum albumin solution at ambient temperature for 1 h. The sections were incubated overnight with primary antibodies targeting SLC8A1 (1:1000, 28447-1-AP, Proteintech, Wuhan, China).

Techniques: Control, Staining, Immunofluorescence, Western Blot, Quantitative RT-PCR, Expressing

Journal: Molecular Cytogenetics

Article Title: SLC8A1 as a novel susceptibility gene in facilitating tendinopathy: insights into its mechanisms from Mendelian randomization and experimental validation

doi: 10.1186/s13039-025-00738-z

Figure Lengend Snippet: MR analysis showed that genetic susceptibility to SLC8A1 eQTL was associated with reduced levels of gamma − glutamylisoleucine

Article Snippet: The sections were permeabilized with 0.1% Triton X-100 in PBS for 15 min. After permeabilization, the sections were blocked using a 5% bovine serum albumin solution at ambient temperature for 1 h. The sections were incubated overnight with primary antibodies targeting SLC8A1 (1:1000, 28447-1-AP, Proteintech, Wuhan, China).

Techniques: